![]() Samples with low field-induced velocity are slightly affected by the magnetic force, thus move along the separation channel (not crossing the OSP) and exit at outlet b. The positions of ISP and OSP are determined by the relative flow-rates of the two inlet and outlet substreams, respectively. The boundary planes between the two inlet and outlet flows are called the inlet splitting plane (ISP) and the outlet splitting plane (OSP), respectively. Samples in the carrier are introduced into one inlet and carriers were introduced into the other inlet with a higher flow-rate to confine samples in a small initial zone for better separation. The parallel set up of separation channel and gravitational force can avoid gravity effect from separation. Typical magnetic SF has two inlets and two outlets, as shown in Fig. Magnetic SF is a new member of SF family for separating magnetically susceptible colloids, and particles. Magnetic separation is fast, simple, and selective. SF is mainly used for preparative applications whereas FFF is mainly used for analytical applications. SF and field-flow fractionation (FFF) are close family of separation techniques for macromolecules, colloids, and particles. In SPLITT fractionation (SF), thin (< 0.5 mm) channels without packing stationary phase are used and different forces are applied perpendicularly to the channel flow for separations. Split-flow thin (SPLITT) fractionation has become useful separation techniques for macromolecules, colloids, and particles. Sample recoveries were 93.1 ± 1.8% in triplicate experiments. The throughput was increased by 18 folds versus early study. Continuous separations of ion-labeled RBC using magnetic SF were successful over 4 hours. This technique also has great potential for cell separation and related analysis. Magnetic SF can provide simple and economical determination of particle susceptibility. The throughput of magnetic SF was approximately 1.8 g/h using our experimental setup. The minimum difference in magnetic susceptibility required for complete separation was about 5.0 × 10 -6. The determined susceptibilities differed by 10% from referenced measurements. Determined susceptibilities of ion-labeled RBC were consistent within 9.6% variations at two magnetic intensities and different flow-rates. ![]() The susceptibilities of Dynabeads determined by SPLITT fractionation (SF) were consistent with those of reference measurement using a superconducting quantum interference device (SQUID) magnetometer. Throughputs were studied at different sample concentrations, magnetic field intensities, and channel flow-rates. Various ion-labeled red blood cells (RBC) were used to study susceptibility determination and throughput parameters for analytical and preparative applications of magnetic SPLITT fractionation (SF), respectively. Reference measurements of magnetic susceptibility were made using a superconducting quantum interference device (SQUID) magnetometer. Magnetic particles, Dynabeads, were used to test this new approach of field-induced velocity for susceptibility determination using magnetic SF at different magnetic field intensities. Particles with different field-induced velocities can be separated into two fractions by adjusting applied magnetic forces and flow-rates at inlets and outlets. Magnetic Split-flow thin (SPLITT) fractionation is a newly developed technique for separating magnetically susceptible particles. ![]()
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